The Clapham laboratory occupies approximately 6000 sq. ft. of space in the Enders Pediatric Research building at Children's Hospital; approximately 2000 sq. ft. are devoted to electrophysiology, containing 8 complete patch clamp setups, and a brain slice recording station. Each setup is provided with a microscope, amplifiers, recording apparatus, computer and manipulators standard for these types of recordings. Another setup is a fura-2 calcium imaging system using an intensified CCD camera. A separate setup is an Olympus Fluoview 1000 inverted 1 and 2 photon confocal microscope adapted for UV laser uncaging. The laboratory also has a total internal relectance fluorescence microscope adapted for biological applications, and a separate Olympus upright Fluoview confocal microscope. A tissue culture room of approximately 500 sq. ft. contains 4 incubators, 3 laminar flow hoods, and microscopes. Another 3000 sq. ft. of space is devoted to protein chemistry and molecular biology. A cold room, two FPLCs, ultracentrifuges, phosphoimager, etc. are all within this laboratory space. Postdoctoral fellows and students have adequate desk and computer space in the laboratory.
As part of the Longwood Medical Area complex, the laboratory has access to substantial core and shared use facilities. The Children's Hospital has core laboratories facilities for oligonucleotides synthesis, peptide and DNA sequencing, transgenic facilities, and procurement of pathological tissues. There are SEM, EM, mass spectrometry and NMR facilities available. The laboratory is also part of the Department of Neurobiology at Harvard Medical School and has access to its machine shop and other core facilities.
David E. Clapham MD, PhD.
Aldo R. Castenada Professor of Cardiovascular Research, Children's Hospital Boston
Professor of Neurobiology and Pediatrics, Harvard Medical School
Investigator, Howard Hughes Medical Institute.
Dr. David Clapham was trained in electrical engineering at the Georgia Institute of Technology and obtained his MD and PhD degrees (with Drs. Louis DeFelice and Robert DeHaan) from Emory University in Atlanta, Georgia. He completed his residency in Internal Medicine at Boston's Brigham and Women's Hospital and his postdoctoral fellowship with Dr. Erwin Neher of the Max Planck Institute in Goettingen Germany. After several years on the faculty at the Mayo Clinic, he moved to Children's Hospital in Boston, where he directs cardiovascular research at Children's Hospital and is Professor of Neurobiology at Harvard Medical School. Dr. Clapham is an investigator of the Howard Hughes Medical Institute.