PUBLICATIONS Publications and Data: - - - - - - - Supplementary Data - - - - - - - - - Papers: - - 2009 - - Hv1 proton channels are required for high-level NADPH oxidase-dependent superoxiEssential role for TRPC5 in amygdala function and fear-related behaviorTRPM1 forms ion channels associated with melanin content in melanocytesIntracellular calcium strongly potentiates agonist-activated TRPC5 channels - - 2008 - - TRPM7 facilitates cholinergic vesicle fusion with the plasma membrane. Citral sensing by transient receptor potential channels in dorsal root ganglion Deletion of Trpm7 Disrupts Embryonic Development and Thymopoiesis Without AlteriTransient Receptor Potential (TRP) ChannelsEvolutionary genomics reveals lineage-specific gene loss and rapid evolution of - - 2007 - - All four CatSper ion channel proteins are required for male fertility and sperm TRPC Canonical (Short) TRP Channel TableTRP Channels Table - TRPA (Ankyrin), TRPP (Polycystins), TRPML (mucolipins)TRPM Channel Family (Melastatin-Related, Long TRPs) TableTRPV Channel Family (Vanilloid) TableKSper, a pH-sensitive K+ current that controls sperm membrane potential. CatSper beta: A novel transmembrane protein in the CatSper channel complexActivating mutation in a mucolipin transient receptor potential channel leads to - - 2006 - - Functional TRPM7 channels accumulate at the plasma membrane in response to fluidWhole-cell patch-clamp measurements of spermatazoa reveal an alkaline-activated International Union of Pharmacology. XLIX. Nomenclature and structure-function rAn Introduction to TRP ChannelsFlavors and skin sensitizers activate specific TRP channels.A voltage-gated proton-selective channel lacking the pore domain.Bisandrographalide from Andrographis Paniculata activates TRPV4 channels The TRPM7 ion channel functions in cholinergic synaptic vesicles and affects tr - - 2005 - - TRPC6 is a glomerular slit diaphragm-associated channel requiredd for normal renTRP channels and mice deficient in TRP channels.TATA-Binding Protein (TBP)-Like Factor (TLF) Is a Functional Regulator of TranscCamphor Activates and Strongly Desensitizes the Transient Receptor Potential VanInternational Union of Pharmacology. XLIX. Nomenclature and structure-function r International Union of Pharmacology. LIV. Nomenclature and Molecular Relationsh - - 2004 - - A Superfamily of Voltage-gated Sodium Channels in BacteriaThe mitochondrial calcium uniporteris a highly selective ion channelA spontaneous, recurrent mutation in Divalent Metal Transporter-1 exposes a calcTRP ion channels in the nervous systemThe voltage-gated Na+ channel NaVBP has a rolein motility, chemotaxis, and pH hRapid vesicular translocation and insertion ofTRP channelsSynGAP-MUPP1-CaMKII Synaptic Complexes Regulatep38 MAP Kinase Activity and NMDA Phosphatidylinositol 3-Kinase Activates ERK in Primary Sensory Neurons and Medi - - 2003 - - Real-Time Imaging of Nuclear Permeation by EGFP in SingleMechanism of Persistent Protein Kinase D1 Translocation and ActivationFormation of novel TRPC channels by complex subunit interactions in embryonic brThe NMDA Receptor Is Coupled to the ERK Pathway by a Direct Interaction betweenTRPC5 is a regulator of hippocampal neurite length and growth cone morphologyFormation of Novel TRPC Channels by Complex Subunit Interactions in Embryonic BrTransient Receptor Potential Ion ChannelsCatSper1 required for evoked Ca2+ entry and control of flagellar function in speTRP channels as cellular sensorsSymmetry, Selectivity, and the 2003 Nobel PrizeTRP CHANNELS A superfamily of voltage-gated sodium channels in bacteria.International Union of Pharmacology. XLI. Compendium of Voltage-Gated Ion ChannInternational Union of Pharmacology. XLIII. Compendium of Voltage-Gated Ion ChaInternational Union of Pharmacology: Approaches to the Nomenclature of Voltage- - - 2002 - - Hot and cold ion channelsStructural characterization of the mouse Girk genesThe TRPM7 channel is inactivated by PIP2 hydrolysisSupplementary data to Xu, Ramsey, Kotecha et alTRPV3 is a calcium-permeable temperature-sensitive cation channel Sorting out MIC, TRP, and CRAC ion channels The cation selectivity filter of the bacterial sodium channel, NaChBac. Modified herpes simplex virus delivery of enhanced GFP into the central nervous The TRP Ion Channel FamilyA voltage-gated ion channel expressed specifically in spermatozoa - - 2001 - - TRP-PLIK, a bifunctional protein with kinase and ion channel activitiesTRPC1 and TRPC5 form a novel cation channel in mammalian brainCaT1 manifests the pore properties of the calcium-release-activated calcium chanHow to lose your hippocampus by working on chloride channelsThe stoichiometry of G-beta/gamma binding G-protein-regulated inwardly rectifyinFundamental Ca2+ signaling mechanisms in mouse dendritic cells: CRAC is the majoEvaluation of the role of I-kach in atrial fibrillation using a mouse knockout mA sperm ion channel required for sperm motility and male fertilityThe TRP ion channel familyA prokaryotic voltage-gated sodium channel - - 2000 - - Function and biochemical evidence for G-protein-gated inwardly rectifying K+ (GIDistinct ion channel classes are expressed in the outer nuclear envelope of T- aA switch mechanism for G-beta/gamma activation of I-kachBrain localization and behavioral impact of the G-protein-gated K+ channel subunICln is essential for cellular and early embryonic viability - - 1999 - - Molecular determinants for subcellular localization of PSD-95 with an interactinMore pieces of the K+ ion channel puzzleFunctional expression and characterization of G-protein gated inwardly rectifyinGIRK4 confers appropriate processing and cell surface localization to G-protein-G-beta/gamma binding increases the open time of Ikach: kinetic evidence for multConformational changes of the in situ nuclear pore complex - - 1998 - - pICln binds to a mammalian homolog of a yeast protein involved in regulation of A novel inward rectifier K+ channel with unique pore propertiesNMDA receptors amplify calcium influx into dendritic spines during associative pActive nuclear import and export is independent of lumenal Ca2+ stores in intactAbnormal heart rate regulation in GIRK4 knockout miceIdentification of native atrial G-protein-regulated inwardly rectifying K+ (GIRKNumber and stoichiometry of subunits in the native atrial G-protein-gated K+ chaLocalization and interaction of epitope-tagged GIRK1 and CIR inward rectifier K+G beta/gamma binding to GIRK4 subunit is critical for G-protein-gated K+ channel - - 1997 - - Ion Channels: Basic Science and Clinical Disease - - 1996 - - Cloning of a Xenopus laevis inwardly rectifying K+ channel subunit that permits Non-selective and G beta/gamma insensitive weaver K+ channelsConformational states of the nuclear pore complex induced by depletion of nuclea - - 1995 - - Diffusion across the nuclear envelope inhibited by depletion of the nuclear Ca2+The G-protein gated atrial K+ channel Ikach is a heterodimer of two inwardly recG-beta/gamma binds directly to the G-protein gated K+ channel, IkachThe G protein beta/gamma subunit transduces the muscarinic receptor signal for CCalcium release from the nucleus by pIns3 Receptor ChannelsCalcium Signalling: Review - - 1994 - - Molecular characterization of a swelling-induced chloride conductance regulatoryRecombinant G-protein beta/gamma subunits activate the muscarinic-gated atrial p - - 1993 - - Optical modifications enabling simultaneous confocal imaging with dyes excited bAcceleration of intracellular calcium waves in xenopus oocytes by calcium influx - - 1992 - - Inositol 1,3,4,5-tetrakisphosphate activates an endothelial Ca2+ permeable channNew mammalian chloride channel identified by expression cloning - - 1991 - - Cloning and expression of a rat cardiac delayed rectifier potassium channelChloride channels in the nuclear membraneSpiral calcium wave propagation and annihilation in xenopus laevis oocytesSubcellular patterns of calcium release determined by G protein specific residue - - 1989 - - Potassium channels in cardiac cells activated by arachidonic acid and phospholipG protein betagamma subunits activate the cardiac muscarinic K channel via PLA2 - - 1988 - - Specificity of action of guanine nucelotide-binding regulatory protein subunits Haemodynamic shear stress activates a K+ current in vascular endothelial cells - - 1987 - - The Beta/Gamma subunits of GTP-binding protein activate the muscarinic K+ channe - - 1985 - - Gamma Aminobutyric acid receptor channels in adrenal chromaffin cells: A patch c - - 1984 - - Development of a delayed outward-rectifying K+ conductance in cultured mouse perTRIFLUOPERAZINE REDUCES INWARD IONIC CURRENTS AND SECRETION BY SEPARATE MECHANISSUBSTANCE P REDUCES ACETYLCHOLINEINDUCED CURRENTS IN ISOLATED BOVINE CHROMAFFIN  Publications Table Supplemental Data RESOURCES TRP Channel Tables TRPC Family TRPM Family TRPV Family Others: TRPA, TRPP, TRPML MTA/Clone Requests Send all forms w/ request: HHMI ShortMTA Reagent Requests ABOUT THE LAB Our Research: Calcium Signalling Channels and Fertility Media: Video Figures The Lab: Lab Facilities Lab Members PRINCIPAL INVESTIGATOR [ Research ] [ E-mail ] David E. Clapham   MD, PhD. Aldo R. Castenada Professor of Cardiovascular Research, Children's Hospital Boston Professor of Neurobiology and Pediatrics, Harvard Medical School Investigator, Howard Hughes Medical Institute. Dr. David Clapham was trained in electrical engineering at the Georgia Institute of Technology and obtained his MD and PhD degrees (with Drs. Louis DeFelice and Robert DeHaan) from Emory University in Atlanta, Georgia. He completed his residency in Internal Medicine at Boston's Brigham and Women's Hospital and his postdoctoral fellowship with Dr. Erwin Neher of the Max Planck Institute in Goettingen Germany. After several years on the faculty at the Mayo Clinic, he moved to Children's Hospital in Boston, where he directs cardiovascular research at Children's Hospital and is Professor of Neurobiology at Harvard Medical School. Dr. Clapham is an investigator of the Howard Hughes Medical Institute. Clapham Lab and friends   POSTDOCTORAL FELLOW [ E-mail ] Nathaniel Blair   PhD . POSTDOCTORAL FELLOW [ E-mail ] Ingrid Carvacho   PhD POSTDOCTORAL FELLOW [ E-mail ] Jean-Ju Chung   PhD DIVISION ADMINISTRATOR [ E-mail ] Edward Cosgrove   PhD ASSOCIATE SCIENTIFIC - RESEARCH / INSTRUCTOR [ E-mail ] Markus Delling   PhD ASSOCIATE SCIENTIFIC - RESEARCH / INSTRUCTOR [ E-mail ] Bimal Desai   PhD POSTDOCTORAL FELLOW [ E-mail ] Julia Doerner   PhD RESEARCH TECHNICIAN [ E-mail ] Svetlana Gapon   Svetlana handles various sensitive duties and maintains the lab's cell cultures. POSTDOCTORAL FELLOW [ E-mail ] Dawei Jiang   PhD POSTDOCTORAL FELLOW [ E-mail ] Jie Jin   PhD RESEACH TECHNICIAN [ E-mail ] Janice Jun   NEUROSCIENCE GRADUATE STUDENT [ E-mail ] J. Stefan Kaczmarek   . ASSISTANT PROFESSOR [ E-mail ] Grigory Krapivinsky   PhD RESEARCH TECHNICIAN [ E-mail ] Luba Krapivinsky   MD/PHD STUDENT, HARVARD-MIT PROGRAM [ E-mail ] Martin V. Kurtev   BBS GRADUATE STUDENT [ E-mail ] Linyu Li   RESEARCH TECHNICIAN [ E-mail ] Yunona Manasian   POSTDOCTORAL FELLOW [ E-mail ] Davide Marini   PhD ADMINISTRATIVE ASSISTANT II [ E-mail ] Michelle Estrada Merry   * Assistant to David E. Clapham, MD, PhD, and the Cardiovascular Research Labs * Administrative Coordinator, Cardiovascular Seminar Series * Fellowship Coordinator, Research Div., Dept. of Cardiology ASSOCIATE SCIENTIFIC - RESEARCH / INSTRUCTOR [ E-mail ] Kiyoshi Miki   PhD ASSOCIATE SCIENTIFIC - RESEARCH / INSTRUCTOR [ E-mail ] Betsy Navarro   PhD RESEARCH TECHNICIAN [ E-mail ] Tina Perivolarakis   ADMINISTRATIVE ASSISTANT [ E-mail ] Carmen Popovici   GLASSWASHING STAFF Glykeria Poutias   POSTDOCTORAL FELLOW [ E-mail ] Antonio Riccio   PhD POSTDOCTORAL FELLOW [ E-mail ] Rajan Sah   MD, PhD POSTDOCTORAL FELLOW [ E-mail ] Stephanie Stotz   PhD POSTDOCTORAL FELLOW [ E-mail ] Tara-Beth Sweet   PhD RESEARCH TECHNICIAN [ E-mail ] Esma Uysal   POSTDOCTORAL FELLOW [ E-mail ] Serdar Uysal   PhD POSTDOCTORAL FELLOW [ E-mail ] Long-Jun Wu   PhD I received my Ph.D. in Biophysics in July 2004, under the supervision of Dr. Tian-Le Xu in University of Science and Technology of China, during which my research focused on the molecular mechanisms of pain at the level of spinal cord. In January 2003, we moved from Uiversity of Science and Technology of China to Institute of Neuroscience, Chinese Academy of Sciences, which is established by Dr. Mu-Ming Poo. I am really proud of being a part of this institute with such a fantastic environment for neuroscience. In November 2004, I went to University of Toronto and started my first postdoctoral training in Dr. Min Zhuo’s lab, where I am working on the pain and memory mechanism, shifting from the level of spinal cord to brain, particularly cingulate cortex, amygdala and hippocampus. Under the support of postdoctoral fellowships from both Canadian Institutes of Health Research and Fragile X Foundation of Canada, I finished various projects on functional studies of NMDA receptors, kainate receptors, GABAA receptors, ATP receptors, Neurabin, FMRP etc in neurons or microglia in brain. In January 2008, I came to Dr. David Clapham lab for my second postdoctoral training, where I hope could be closer to molecular mechanisms of ion channel functions. RESEARCH TECHNICIAN [ E-mail ] Linlin Zhao   POSTDOCTORAL FELLOW [ E-mail ] Hao Zhou   PhD CONTACT US E-mail General Inquiries Michelle Estrada Merry Address: David E. Clapham MD, PhD. Howard Hughes Medical Institute Department of Cardiology, Children's Hospital Department of Neurobiology, Harvard Medical School 1309 Enders Research Building 320 Longwood Avenue Boston, MA 02115 Phone: 617-919-2680 FAX: 617-731-0787 E-Mail: David E Clapham NEWS Welcome to the new Clapham Lab website! Feedback is welcome; direct to General Inquiries. TRP Channel Tables available in Resources. MTA (Material) / Clone Request forms available in Resources. Administration Panel

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