PUBLICATIONS Publications and Data: - - - - - - - Supplementary Data - - - - - - - - - Papers: - - 2012 - - The voltage-gated proton channel Hv1 enhances brain damage from ischemic strokeCalpain cleaves and activates the TRPC5 channel to participate in semaphorin 3A- - - 2011 - - A novel gene required for male fertility and functional CATSPER channel formatioAncestral Ca2+ signaling machinery in early animal and fungal evolutionATP-activated P2X2 current in mouse spermatozoa.Transient receptor potential cation channel, subfamily C, member 5 (TRPC5) is a POST, Partner of STIM1, targets STIM1 to multiple transporters.Melanopsin signalling in mammalian iris and retina. The control of male fertility by spermatozoan ion channels. The channel kinase, TRPM7, is required for early embryonic development A thermodynamic framework for understanding temperature sensing by transient rec - - 2010 - - A keratinocyte TRP channel controls hair morphogenesis and skin barrier formatioAn aqueous H+ permeation pathway in the voltage-gated proton channel Hv1International Union of Basic and Clinical Pharmacology. LXXVI. Current Progress - - 2009 - - Hv1 proton channels are required for high-level NADPH oxidase-dependent superoxiEssential role for TRPC5 in amygdala function and fear-related behaviorTRPM1 forms ion channels associated with melanin content in melanocytesIntracellular calcium strongly potentiates agonist-activated TRPC5 channelsMammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebr - - 2008 - - TRPM7 facilitates cholinergic vesicle fusion with the plasma membrane. Citral sensing by transient receptor potential channels in dorsal root ganglion Deletion of Trpm7 Disrupts Embryonic Development and Thymopoiesis Without AlteriTransient Receptor Potential (TRP) ChannelsEvolutionary genomics reveals lineage-specific gene loss and rapid evolution of - - 2007 - - All four CatSper ion channel proteins are required for male fertility and sperm TRPC Canonical (Short) TRP Channel TableTRP Channels Table - TRPA (Ankyrin), TRPP (Polycystins), TRPML (mucolipins)TRPM Channel Family (Melastatin-Related, Long TRPs) TableTRPV Channel Family (Vanilloid) TableKSper, a pH-sensitive K+ current that controls sperm membrane potential. CatSper beta: A novel transmembrane protein in the CatSper channel complexActivating mutation in a mucolipin transient receptor potential channel leads to Calcium signalingSnapShot: Mammalian TRP Channels. - - 2006 - - Functional TRPM7 channels accumulate at the plasma membrane in response to fluidWhole-cell patch-clamp measurements of spermatazoa reveal an alkaline-activated International Union of Pharmacology. XLIX. Nomenclature and structure-function rAn Introduction to TRP ChannelsFlavors and skin sensitizers activate specific TRP channels.A voltage-gated proton-selective channel lacking the pore domain.Bisandrographalide from Andrographis Paniculata activates TRPV4 channels The TRPM7 ion channel functions in cholinergic synaptic vesicles and affects tr - - 2005 - - TRPC6 is a glomerular slit diaphragm-associated channel requiredd for normal renTRP channels and mice deficient in TRP channels.TATA-Binding Protein (TBP)-Like Factor (TLF) Is a Functional Regulator of TranscCamphor Activates and Strongly Desensitizes the Transient Receptor Potential VanInternational Union of Pharmacology. XLIX. Nomenclature and structure-function r International Union of Pharmacology. LIV. Nomenclature and Molecular Relationsh - - 2004 - - A Superfamily of Voltage-gated Sodium Channels in BacteriaThe mitochondrial calcium uniporteris a highly selective ion channelA spontaneous, recurrent mutation in Divalent Metal Transporter-1 exposes a calcTRP ion channels in the nervous systemThe voltage-gated Na+ channel NaVBP has a rolein motility, chemotaxis, and pH hRapid vesicular translocation and insertion ofTRP channelsSynGAP-MUPP1-CaMKII Synaptic Complexes Regulatep38 MAP Kinase Activity and NMDA Phosphatidylinositol 3-Kinase Activates ERK in Primary Sensory Neurons and Medi - - 2003 - - Real-Time Imaging of Nuclear Permeation by EGFP in SingleMechanism of Persistent Protein Kinase D1 Translocation and ActivationFormation of novel TRPC channels by complex subunit interactions in embryonic brThe NMDA Receptor Is Coupled to the ERK Pathway by a Direct Interaction betweenTRPC5 is a regulator of hippocampal neurite length and growth cone morphologyFormation of Novel TRPC Channels by Complex Subunit Interactions in Embryonic BrTransient Receptor Potential Ion ChannelsCatSper1 required for evoked Ca2+ entry and control of flagellar function in speTRP channels as cellular sensorsSymmetry, Selectivity, and the 2003 Nobel PrizeTRP CHANNELS A superfamily of voltage-gated sodium channels in bacteria.International Union of Pharmacology. XLI. Compendium of Voltage-Gated Ion ChannInternational Union of Pharmacology. XLIII. Compendium of Voltage-Gated Ion ChaInternational Union of Pharmacology: Approaches to the Nomenclature of Voltage- - - 2002 - - Hot and cold ion channelsStructural characterization of the mouse Girk genesThe TRPM7 channel is inactivated by PIP2 hydrolysisSupplementary data to Xu, Ramsey, Kotecha et alTRPV3 is a calcium-permeable temperature-sensitive cation channel Sorting out MIC, TRP, and CRAC ion channels The cation selectivity filter of the bacterial sodium channel, NaChBac. Modified herpes simplex virus delivery of enhanced GFP into the central nervous The TRP Ion Channel FamilyA voltage-gated ion channel expressed specifically in spermatozoa - - 2001 - - TRP-PLIK, a bifunctional protein with kinase and ion channel activitiesTRPC1 and TRPC5 form a novel cation channel in mammalian brainCaT1 manifests the pore properties of the calcium-release-activated calcium chanHow to lose your hippocampus by working on chloride channelsThe stoichiometry of G-beta/gamma binding G-protein-regulated inwardly rectifyinFundamental Ca2+ signaling mechanisms in mouse dendritic cells: CRAC is the majoEvaluation of the role of I-kach in atrial fibrillation using a mouse knockout mA sperm ion channel required for sperm motility and male fertilityThe TRP ion channel familyA prokaryotic voltage-gated sodium channel - - 2000 - - Function and biochemical evidence for G-protein-gated inwardly rectifying K+ (GIDistinct ion channel classes are expressed in the outer nuclear envelope of T- aA switch mechanism for G-beta/gamma activation of I-kachBrain localization and behavioral impact of the G-protein-gated K+ channel subunICln is essential for cellular and early embryonic viability - - 1999 - - Molecular determinants for subcellular localization of PSD-95 with an interactinMore pieces of the K+ ion channel puzzleFunctional expression and characterization of G-protein gated inwardly rectifyinGIRK4 confers appropriate processing and cell surface localization to G-protein-G-beta/gamma binding increases the open time of Ikach: kinetic evidence for multConformational changes of the in situ nuclear pore complex - - 1998 - - pICln binds to a mammalian homolog of a yeast protein involved in regulation of A novel inward rectifier K+ channel with unique pore propertiesNMDA receptors amplify calcium influx into dendritic spines during associative pActive nuclear import and export is independent of lumenal Ca2+ stores in intactAbnormal heart rate regulation in GIRK4 knockout miceIdentification of native atrial G-protein-regulated inwardly rectifying K+ (GIRKNumber and stoichiometry of subunits in the native atrial G-protein-gated K+ chaLocalization and interaction of epitope-tagged GIRK1 and CIR inward rectifier K+G beta/gamma binding to GIRK4 subunit is critical for G-protein-gated K+ channel - - 1997 - - Ion Channels: Basic Science and Clinical Disease - - 1996 - - Cloning of a Xenopus laevis inwardly rectifying K+ channel subunit that permits Non-selective and G beta/gamma insensitive weaver K+ channelsConformational states of the nuclear pore complex induced by depletion of nuclea - - 1995 - - Diffusion across the nuclear envelope inhibited by depletion of the nuclear Ca2+The G-protein gated atrial K+ channel Ikach is a heterodimer of two inwardly recG-beta/gamma binds directly to the G-protein gated K+ channel, IkachThe G protein beta/gamma subunit transduces the muscarinic receptor signal for CCalcium release from the nucleus by pIns3 Receptor ChannelsCalcium Signalling: Review - - 1994 - - Molecular characterization of a swelling-induced chloride conductance regulatoryRecombinant G-protein beta/gamma subunits activate the muscarinic-gated atrial p - - 1993 - - Optical modifications enabling simultaneous confocal imaging with dyes excited bAcceleration of intracellular calcium waves in xenopus oocytes by calcium influx - - 1992 - - Inositol 1,3,4,5-tetrakisphosphate activates an endothelial Ca2+ permeable channNew mammalian chloride channel identified by expression cloning - - 1991 - - Cloning and expression of a rat cardiac delayed rectifier potassium channelChloride channels in the nuclear membraneSpiral calcium wave propagation and annihilation in xenopus laevis oocytesSubcellular patterns of calcium release determined by G protein specific residue - - 1989 - - Potassium channels in cardiac cells activated by arachidonic acid and phospholipG protein betagamma subunits activate the cardiac muscarinic K channel via PLA2 - - 1988 - - Specificity of action of guanine nucelotide-binding regulatory protein subunits Haemodynamic shear stress activates a K+ current in vascular endothelial cells - - 1987 - - The Beta/Gamma subunits of GTP-binding protein activate the muscarinic K+ channe - - 1985 - - Gamma Aminobutyric acid receptor channels in adrenal chromaffin cells: A patch c - - 1984 - - Development of a delayed outward-rectifying K+ conductance in cultured mouse perTRIFLUOPERAZINE REDUCES INWARD IONIC CURRENTS AND SECRETION BY SEPARATE MECHANISSUBSTANCE P REDUCES ACETYLCHOLINEINDUCED CURRENTS IN ISOLATED BOVINE CHROMAFFIN  Publications Table Supplemental Data RESOURCES TRP Channel Tables TRPC Family TRPM Family TRPV Family Others: TRPA, TRPP, TRPML Clone/Reagents Requests Materials request ABOUT THE LAB Our Research: Ion Channels Media: Video Figures The Lab: Lab Facilities Lab Members eurotransmitters, hormones, and pharmacologic agents act by binding specific receptors or ion channels on the cell surface. We are interested in the structure and function of ion channels, their physiological function, and the signal transduction pathways with which they interact. Molecules in the cell membrane that relay hormonal and neurotransmitter signals bind to receptors on the cell surface (M2, M3) that initiate signalling cascades involving TRP or potassium channels, or calcium release. M2: Muscarinic type 2 acetylcholine (ACh) receptor. M3: Muscarinic type 3 ACh receptor. G: G protein. GIRK: G protein gated inward rectifier channel subunit. PLC: phospholipase C or PIP2 phosphodiesterase. InsP3: Inositol (1,4,5) triphosphate. GTP: guanosine triphosphate. GDP: guanosine diphosphate. DAG: diacylglycerol. TRP: transient receptor potential channel. K+: potassium selective channel. IP3R: InsP3 receptor. SERCA: smooth endoplasmic reticulum Ca2+ ATPase pump. + indicates stimulatory regulation. - indicates inhibition. CONTACT US E-mail General Inquiries Michelle E. Merry Address: David E. Clapham MD, PhD. Howard Hughes Medical Institute Department of Cardiology, Children's Hospital Department of Neurobiology, Harvard Medical School 1309 Enders Research Building 320 Longwood Avenue Boston, MA 02115 Phone: 617-919-2680 FAX: 617-731-0787 E-Mail: David E Clapham NEWS MTA (Material) / Clone Request available in Resources. Welcome to the new Clapham Lab website! Feedback is welcome; direct to General Inquiries. TRP Channel Tables available in Resources. Administration Panel

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